The Sanger's Method Of DNA Sequencing
The Sanger’s Method of DNA sequencing, also known as chain termination or dideoxy sequencing, is done by adding a hydrogen group to a single strand of DNA in order to stop the nucleotides from replicating and to make it possible to effectively sequence the strand. Sanger’s Method of DNA sequencing was introduced in 1974 and is now the current accepted standard in DNA sequencing as it is thought to be the most practical method.
Knowing the sequence of the nucleotides of a strand of DNA allows scientists to duplicate the strands and identify gene mutations that cause differences, deformations, and even diseases. Ongoing DNA research sine the inception of Sanger’s Method of DNA sequencing has brought about what once would have been considered medical and scientific miracles. There is no doubt that because of Sanger’s Method of DNA sequencing future genetic research will produce results that we would never have thought possible in our lifetime.
Sanger’s Method of DNA sequencing is done by separating a DNA strand into single strands by heating it at high temperatures. A primer is then attached to one of the DNA strands in a manner in which the 3’ end of the primer is placed at the location on the DNA that is to be studied. The primer will then be marked either fluorescently or radioactively in order to be easily found during the final stages of the method.
The Sanger’s Method of DNA sequencing then places these strands into four tubes, each labeled for one type of the nucleotide of the DNA. These tubes are then analyzed to determine the sequence of the nucleotides of the strand of the DNA. The results of the Sanger’s Method of DNA sequencing analysis are then assembled into a computer program, commonly 454 nucleotides long. The assembly is then labeled and entered into a database service to be shared with scientist around the world who are also using Sanger’s Method of DNA sequencing.
Sanger’s Method of DNA sequencing is very costly and the throughput is quite slow for sequencing long strings of DNA. It would take months or longer to run the DNA of a human through the Sanger’s Method of DNA sequencing. A new method has recently been introduced that could very well outshine Sanger’s Method of DNA sequencing because it is very fast to align the DNA strand and sequence it, and it is inexpensive. It is thought that this method will overtake any contract on Sanger’s Method of DNA sequencing and soon become the new standard in DNA sequencing.
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